Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Article Title: Crosstalk Between Substance P and Calcitonin Gene-Related Peptide During Heterotopic Ossification in Murine Achilles Tendon

doi: 10.1002/jor.23833

Figure Lengend Snippet: SP delivery induced HO in Achilles at week 6 post-operation according to the microCT scans. Co-delivery of SP and CGRP did not exhibit any HO. (A) Representative microCT images of the treated legs. Scale bars measure 5mm. Arrows point to the HO nodules around the Achilles. (B) HO around the calcaneus and the Achilles. Bars represent the average HO volume ± SEM. n = 6 per group. (C) HO around the calcaneus and the Achilles. Bars represent the bone volume (BV)/total volume (TV) ratio ± SEM. Control animals did not show any HO around the tendon, but the micro hole drilled through the calcaneus was healed. SP induced significant HO (p < 0.001) around the Achilles, while CGRP did not have a significant effect (p = 0.9 compared to the controls, p < 0.01 compared to SP-delivered tendons). Co-delivery of SP and CGRP did not induce HO (p μ 0.001 compared to SP-delivered tendons).

Article Snippet: Three-dimensional reconstruction of the lateral projections were performed using the Scanco software from the raw files and the resulting volumes were processed with the Scanco microCT 3D morphometry and densitometry software, operated in an open VMS environment.

Techniques:

Journal: BMC Oral Health

Article Title: Low-level laser therapy prevents medication-related osteonecrosis of the jaw-like lesions via IL-1RA-mediated primary gingival wound healing

doi: 10.1186/s12903-022-02678-1

Figure Lengend Snippet: Effects of LLLT on MRONJ-like mouse model. A The study's experimental protocols are depicted in this diagram. B Representative intraoral photos. (Ctrl: non-drug treatment group; Zol: treated with Zol group; Zol + LLLT: treated with Zol and LLLT group). Green dotted lines represent unhealed gingiva. C MicroCT reconstructed 3D images of tooth sockets. Green dotted lines represent tooth sockets. D , E Quantification of BMD and BV/TV in each group. F H&E staining shows tooth extraction sockets-wound healing in each group. Black dotted boxes represent magnified boxed regions. Green dotted lines represent unhealed gingiva. Green dot lines represent necrotic bones. Scale bar = 200 μm (upper), scale bar = 100 μm (lower). G H&E staining shows the human gingiva of healthy and MRONJ patients. Black dotted boxes represent magnified boxed regions. Black arrows represent inflammatory cells. Scale bar = 200 μm (upper), scale bar = 50 μm (lower). H Masson’s trichrome staining shows tooth extraction sockets-wound healing in each group. Yellow dotted boxes represent magnified boxed regions. Scale bar = 200 μm (upper), scale bar = 100 μm (lower). I Masson’s trichrome staining shows collagen arrangement in human gingiva tissues. Yellow dotted boxes represent magnified boxed regions. Scale bar = 200 μm (upper), scale bar = 100 μm (lower). (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001)

Article Snippet: The area of interest was chosen, and bone mineral density (BMD) and bone volume/total volume (BV/TV) were quantified by three-dimensional (3D) bone morphometric software (SIEMENS, Munich, Germany).

Techniques: Staining

Journal: BMC Oral Health

Article Title: Low-level laser therapy prevents medication-related osteonecrosis of the jaw-like lesions via IL-1RA-mediated primary gingival wound healing

doi: 10.1186/s12903-022-02678-1

Figure Lengend Snippet: Application of IL-1RA NAb impairs the capacity of LLLT to promote gingival wound healing. A The study's experimental protocols are depicted in this diagram. B Representative intraoral photos. (Ctrl: non-drug treatment, but treated with negative control antibodies group; Zol: treated with Zol and negative control antibodies group; Zol + LLLT: treated with Zol, LLLT, and negative control antibodies group; Zol + LLLT + IL-1RA: treated with Zol, LLLT, and IL-1RA NAb group). Green dotted lines represent unhealed gingiva. C MicroCT reconstructed 3D images of tooth sockets. Green dotted lines represent tooth sockets. D , E Quantification of BMD and BV/TV in each group. F H&E staining shows tooth extraction sockets-wound healing in each group. Black dotted boxes represent magnified boxed regions. Green dotted lines represent unhealed gingiva. Scale bar = 200 μm (upper), scale bar = 100 μm (lower). G , H Images of IL-1β and TNF-α IHC staining of the mouse gingival wound. Scale bar = 100 μm. (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001)

Article Snippet: The area of interest was chosen, and bone mineral density (BMD) and bone volume/total volume (BV/TV) were quantified by three-dimensional (3D) bone morphometric software (SIEMENS, Munich, Germany).

Techniques: Negative Control, Staining, Immunohistochemistry